Development of a genotyping microarray for Usher syndrome
Identifieur interne : 001546 ( Main/Exploration ); précédent : 001545; suivant : 001547Development of a genotyping microarray for Usher syndrome
Auteurs : Frans P M. Cremers [Pays-Bas] ; William J. Kimberling [États-Unis] ; Maigi Külm [Estonie] ; Arjan P. De Brouwer [Pays-Bas] ; Erwin Van Wijk [Pays-Bas] ; Heleen Te Brinke [Pays-Bas] ; Cor W R J. Cremers [Pays-Bas] ; Lies H. Hoefsloot [Pays-Bas] ; Sandro Banfi [Italie] ; Francesca Simonelli [Italie] ; Johannes C. Fleischhauer [Suisse] ; Wolfgang Berger [Suisse] ; Phil M. Kelley [États-Unis] ; Elene Haralambous [Royaume-Uni] ; Maria Bitner-Glindzicz [Royaume-Uni] ; Andrew R. Webster [Royaume-Uni] ; Zubin Saihan [Royaume-Uni] ; Elfride De Baere [Belgique] ; Bart P. Leroy [Belgique] ; Giuliana Silvestri [Royaume-Uni] ; Gareth J. Mckay [Royaume-Uni] ; Robert K. Koenekoop [Canada] ; Jose M. Millan [Espagne] ; Thomas Rosenberg [Danemark] ; Tarja Joensuu [Finlande] ; Eeva-Marja Sankila [Finlande] ; Dominique Weil [France] ; Mike D. Weston [États-Unis] ; Bernd Wissinger [Allemagne] ; Hannie Kremer [Belgique]Source :
- Journal of Medical Genetics [ 0022-2593 ] ; 2007-02.
English descriptors
- KwdEn :
- APEX, arrayed primer extension, RP, retinitis pigmentosa, SSCP, single strand conformation polymorphism, USH, Usher syndrome, USHA, atypical USH, Usher syndrome, arRP, autosomal recessive retinitis pigmentosa, arrayed primer extension, mutation analysis, retinitis pigmentosa, sensorineural deafness.
Abstract
Background: Usher syndrome, a combination of retinitis pigmentosa (RP) and sensorineural hearing loss with or without vestibular dysfunction, displays a high degree of clinical and genetic heterogeneity. Three clinical subtypes can be distinguished, based on the age of onset and severity of the hearing impairment, and the presence or absence of vestibular abnormalities. Thus far, eight genes have been implicated in the syndrome, together comprising 347 protein-coding exons. Methods: To improve DNA diagnostics for patients with Usher syndrome, we developed a genotyping microarray based on the arrayed primer extension (APEX) method. Allele-specific oligonucleotides corresponding to all 298 Usher syndrome-associated sequence variants known to date, 76 of which are novel, were arrayed. Results: Approximately half of these variants were validated using original patient DNAs, which yielded an accuracy of >98%. The efficiency of the Usher genotyping microarray was tested using DNAs from 370 unrelated European and American patients with Usher syndrome. Sequence variants were identified in 64/140 (46%) patients with Usher syndrome type I, 45/189 (24%) patients with Usher syndrome type II, 6/21 (29%) patients with Usher syndrome type III and 6/20 (30%) patients with atypical Usher syndrome. The chip also identified two novel sequence variants, c.400C>T (p.R134X) in PCDH15 and c.1606T>C (p.C536S) in USH2A. Conclusion: The Usher genotyping microarray is a versatile and affordable screening tool for Usher syndrome. Its efficiency will improve with the addition of novel sequence variants with minimal extra costs, making it a very useful first-pass screening tool.
Url:
DOI: 10.1136/jmg.2006.044784
Affiliations:
- Allemagne, Belgique, Canada, Danemark, Espagne, Estonie, Finlande, France, Italie, Pays-Bas, Royaume-Uni, Suisse, États-Unis
- Angleterre, Bade-Wurtemberg, Canton de Zurich, District de Tübingen, Grand Londres, Gueldre, Nebraska, Québec, Uusimaa
- Helsinki, Londres, Montréal, Nimègue, Paris, Tübingen, Zurich
- Université d'Helsinki, Université de Zurich
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Le document en format XML
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Cremers</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Human Genetics, and Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Kimberling, William J" sort="Kimberling, William J" uniqKey="Kimberling W" first="William J" last="Kimberling">William J. 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De Brouwer</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Human Genetics, and Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Van Wijk, Erwin" sort="Van Wijk, Erwin" uniqKey="Van Wijk E" first="Erwin" last="Van Wijk">Erwin Van Wijk</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Otorhinolaryngology, Radboud University Nijmegen Medical Centre, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Te Brinke, Heleen" sort="Te Brinke, Heleen" uniqKey="Te Brinke H" first="Heleen" last="Te Brinke">Heleen Te Brinke</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Otorhinolaryngology, Radboud University Nijmegen Medical Centre, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Cremers, Cor W R J" sort="Cremers, Cor W R J" uniqKey="Cremers C" first="Cor W R J" last="Cremers">Cor W R J. Cremers</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Otorhinolaryngology, Radboud University Nijmegen Medical Centre, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Hoefsloot, Lies H" sort="Hoefsloot, Lies H" uniqKey="Hoefsloot L" first="Lies H" last="Hoefsloot">Lies H. Hoefsloot</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Human Genetics, and Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Banfi, Sandro" sort="Banfi, Sandro" uniqKey="Banfi S" first="Sandro" last="Banfi">Sandro Banfi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Téléthon Institute of Genetics and Medicine, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Simonelli, Francesca" sort="Simonelli, Francesca" uniqKey="Simonelli F" first="Francesca" last="Simonelli">Francesca Simonelli</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Ophthalmology, Second University of Naples, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Fleischhauer, Johannes C" sort="Fleischhauer, Johannes C" uniqKey="Fleischhauer J" first="Johannes C" last="Fleischhauer">Johannes C. Fleischhauer</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Ophthalmology, University Hospital Zurich, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Berger, Wolfgang" sort="Berger, Wolfgang" uniqKey="Berger W" first="Wolfgang" last="Berger">Wolfgang Berger</name><affiliation wicri:level="4"><country xml:lang="fr">Suisse</country><wicri:regionArea>Division of Medical Molecular Genetics and Gene Diagnostics, Institute of Medical Genetics, University of Zurich, Zurich</wicri:regionArea><orgName type="university">Université de Zurich</orgName><placeName><settlement type="city">Zurich</settlement><region nuts="3" type="region">Canton de Zurich</region></placeName></affiliation></author><author><name sortKey="Kelley, Phil M" sort="Kelley, Phil M" uniqKey="Kelley P" first="Phil M" last="Kelley">Phil M. Kelley</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Usher Syndrome Center, Boys Town National Research Hospital, Omaha, Nebraska</wicri:regionArea><placeName><region type="state">Nebraska</region></placeName></affiliation></author><author><name sortKey="Haralambous, Elene" sort="Haralambous, Elene" uniqKey="Haralambous E" first="Elene" last="Haralambous">Elene Haralambous</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Clinical and Molecular Genetics Unit, Institute of Child Health, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Bitner Glindzicz, Maria" sort="Bitner Glindzicz, Maria" uniqKey="Bitner Glindzicz M" first="Maria" last="Bitner-Glindzicz">Maria Bitner-Glindzicz</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Clinical and Molecular Genetics Unit, Institute of Child Health, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Webster, Andrew R" sort="Webster, Andrew R" uniqKey="Webster A" first="Andrew R" last="Webster">Andrew R. Webster</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Molecular Genetics, Institute of Ophthalmology, UCL, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Saihan, Zubin" sort="Saihan, Zubin" uniqKey="Saihan Z" first="Zubin" last="Saihan">Zubin Saihan</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Molecular Genetics, Institute of Ophthalmology, UCL, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="De Baere, Elfride" sort="De Baere, Elfride" uniqKey="De Baere E" first="Elfride" last="De Baere">Elfride De Baere</name><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Center for Medical Genetics, Ghent University Hospital, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Leroy, Bart P" sort="Leroy, Bart P" uniqKey="Leroy B" first="Bart P" last="Leroy">Bart P. Leroy</name><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Ophthalmology, Ghent University Hospital, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Silvestri, Giuliana" sort="Silvestri, Giuliana" uniqKey="Silvestri G" first="Giuliana" last="Silvestri">Giuliana Silvestri</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Vision Science, School of Biomedical Science, Queen’s University Belfast, Belfast, Northern Ireland</wicri:regionArea><wicri:noRegion>Northern Ireland</wicri:noRegion></affiliation></author><author><name sortKey="Mckay, Gareth J" sort="Mckay, Gareth J" uniqKey="Mckay G" first="Gareth J" last="Mckay">Gareth J. Mckay</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Vision Science, School of Biomedical Science, Queen’s University Belfast, Belfast, Northern Ireland</wicri:regionArea><wicri:noRegion>Northern Ireland</wicri:noRegion></affiliation></author><author><name sortKey="Koenekoop, Robert K" sort="Koenekoop, Robert K" uniqKey="Koenekoop R" first="Robert K" last="Koenekoop">Robert K. Koenekoop</name><affiliation wicri:level="3"><country xml:lang="fr">Canada</country><wicri:regionArea>Ocular Genetics Laboratory, McGill University Health Center, Montreal</wicri:regionArea><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Millan, Jose M" sort="Millan, Jose M" uniqKey="Millan J" first="Jose M" last="Millan">Jose M. Millan</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Unidad de Genetica y Diagnostico Prenatal, Hospital Universitario La Fe, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Rosenberg, Thomas" sort="Rosenberg, Thomas" uniqKey="Rosenberg T" first="Thomas" last="Rosenberg">Thomas Rosenberg</name><affiliation wicri:level="1"><country xml:lang="fr">Danemark</country><wicri:regionArea>Gordon Norrie Centre for Genetic Eye Diseases, National Eye Clinic for the Visually Impaired, Hellerup</wicri:regionArea><wicri:noRegion>Hellerup</wicri:noRegion></affiliation></author><author><name sortKey="Joensuu, Tarja" sort="Joensuu, Tarja" uniqKey="Joensuu T" first="Tarja" last="Joensuu">Tarja Joensuu</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>The Folkhalsan Institute of Genetics, Biomedicum Helsinki, and Department of Ophthalmology, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Sankila, Eeva Marja" sort="Sankila, Eeva Marja" uniqKey="Sankila E" first="Eeva-Marja" last="Sankila">Eeva-Marja Sankila</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>The Folkhalsan Institute of Genetics, Biomedicum Helsinki, and Department of Ophthalmology, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Weil, Dominique" sort="Weil, Dominique" uniqKey="Weil D" first="Dominique" last="Weil">Dominique Weil</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Unité de Génétique des Deficits Sensoriels, Institut Pasteur, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Weston, Mike D" sort="Weston, Mike D" uniqKey="Weston M" first="Mike D" last="Weston">Mike D. Weston</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Usher Syndrome Center, Boys Town National Research Hospital, Omaha, Nebraska</wicri:regionArea><placeName><region type="state">Nebraska</region></placeName></affiliation></author><author><name sortKey="Wissinger, Bernd" sort="Wissinger, Bernd" uniqKey="Wissinger B" first="Bernd" last="Wissinger">Bernd Wissinger</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Molecular Genetics Laboratory, University Eye Hospital, Tübingen</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region><settlement type="city">Tübingen</settlement></placeName></affiliation></author><author><name sortKey="Kremer, Hannie" sort="Kremer, Hannie" uniqKey="Kremer H" first="Hannie" last="Kremer">Hannie Kremer</name><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Center for Medical Genetics, Ghent University Hospital, Ghent</wicri:regionArea><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Medical Genetics</title><title level="j" type="abbrev">J Med Genet</title><idno type="ISSN">0022-2593</idno><idno type="eISSN">1468-6244</idno><imprint><publisher>BMJ Publishing Group Ltd</publisher><date type="published" when="2007-02">2007-02</date><biblScope unit="volume">44</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="153">153</biblScope></imprint><idno type="ISSN">0022-2593</idno></series><idno type="istex">85BCC45536CF1BDE4E585D34AB33ED062254F920</idno><idno type="DOI">10.1136/jmg.2006.044784</idno><idno type="href">jmedgenet-44-153.pdf</idno><idno type="PMID">16963483</idno><idno type="PII">1468-6422</idno><idno type="local">0440153</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2593</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>APEX, arrayed primer extension</term><term>RP, retinitis pigmentosa</term><term>SSCP, single strand conformation polymorphism</term><term>USH, Usher syndrome</term><term>USHA, atypical USH</term><term>Usher syndrome</term><term>arRP, autosomal recessive retinitis pigmentosa</term><term>arrayed primer extension</term><term>mutation analysis</term><term>retinitis pigmentosa</term><term>sensorineural deafness</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Usher syndrome, a combination of retinitis pigmentosa (RP) and sensorineural hearing loss with or without vestibular dysfunction, displays a high degree of clinical and genetic heterogeneity. Three clinical subtypes can be distinguished, based on the age of onset and severity of the hearing impairment, and the presence or absence of vestibular abnormalities. Thus far, eight genes have been implicated in the syndrome, together comprising 347 protein-coding exons. Methods: To improve DNA diagnostics for patients with Usher syndrome, we developed a genotyping microarray based on the arrayed primer extension (APEX) method. Allele-specific oligonucleotides corresponding to all 298 Usher syndrome-associated sequence variants known to date, 76 of which are novel, were arrayed. Results: Approximately half of these variants were validated using original patient DNAs, which yielded an accuracy of >98%. The efficiency of the Usher genotyping microarray was tested using DNAs from 370 unrelated European and American patients with Usher syndrome. Sequence variants were identified in 64/140 (46%) patients with Usher syndrome type I, 45/189 (24%) patients with Usher syndrome type II, 6/21 (29%) patients with Usher syndrome type III and 6/20 (30%) patients with atypical Usher syndrome. The chip also identified two novel sequence variants, c.400C>T (p.R134X) in PCDH15 and c.1606T>C (p.C536S) in USH2A. Conclusion: The Usher genotyping microarray is a versatile and affordable screening tool for Usher syndrome. Its efficiency will improve with the addition of novel sequence variants with minimal extra costs, making it a very useful first-pass screening tool.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Belgique</li><li>Canada</li><li>Danemark</li><li>Espagne</li><li>Estonie</li><li>Finlande</li><li>France</li><li>Italie</li><li>Pays-Bas</li><li>Royaume-Uni</li><li>Suisse</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Bade-Wurtemberg</li><li>Canton de Zurich</li><li>District de Tübingen</li><li>Grand Londres</li><li>Gueldre</li><li>Nebraska</li><li>Québec</li><li>Uusimaa</li></region><settlement><li>Helsinki</li><li>Londres</li><li>Montréal</li><li>Nimègue</li><li>Paris</li><li>Tübingen</li><li>Zurich</li></settlement><orgName><li>Université d'Helsinki</li><li>Université de Zurich</li></orgName></list><tree><country name="Pays-Bas"><region name="Gueldre"><name sortKey="Cremers, Frans P M" sort="Cremers, Frans P M" uniqKey="Cremers F" first="Frans P M" last="Cremers">Frans P M. Cremers</name></region><name sortKey="Cremers, Cor W R J" sort="Cremers, Cor W R J" uniqKey="Cremers C" first="Cor W R J" last="Cremers">Cor W R J. Cremers</name><name sortKey="De Brouwer, Arjan P" sort="De Brouwer, Arjan P" uniqKey="De Brouwer A" first="Arjan P" last="De Brouwer">Arjan P. De Brouwer</name><name sortKey="Hoefsloot, Lies H" sort="Hoefsloot, Lies H" uniqKey="Hoefsloot L" first="Lies H" last="Hoefsloot">Lies H. Hoefsloot</name><name sortKey="Te Brinke, Heleen" sort="Te Brinke, Heleen" uniqKey="Te Brinke H" first="Heleen" last="Te Brinke">Heleen Te Brinke</name><name sortKey="Van Wijk, Erwin" sort="Van Wijk, Erwin" uniqKey="Van Wijk E" first="Erwin" last="Van Wijk">Erwin Van Wijk</name></country><country name="États-Unis"><region name="Nebraska"><name sortKey="Kimberling, William J" sort="Kimberling, William J" uniqKey="Kimberling W" first="William J" last="Kimberling">William J. Kimberling</name></region><name sortKey="Kelley, Phil M" sort="Kelley, Phil M" uniqKey="Kelley P" first="Phil M" last="Kelley">Phil M. Kelley</name><name sortKey="Weston, Mike D" sort="Weston, Mike D" uniqKey="Weston M" first="Mike D" last="Weston">Mike D. Weston</name></country><country name="Estonie"><noRegion><name sortKey="Kulm, Maigi" sort="Kulm, Maigi" uniqKey="Kulm M" first="Maigi" last="Külm">Maigi Külm</name></noRegion></country><country name="Italie"><noRegion><name sortKey="Banfi, Sandro" sort="Banfi, Sandro" uniqKey="Banfi S" first="Sandro" last="Banfi">Sandro Banfi</name></noRegion><name sortKey="Simonelli, Francesca" sort="Simonelli, Francesca" uniqKey="Simonelli F" first="Francesca" last="Simonelli">Francesca Simonelli</name></country><country name="Suisse"><noRegion><name sortKey="Fleischhauer, Johannes C" sort="Fleischhauer, Johannes C" uniqKey="Fleischhauer J" first="Johannes C" last="Fleischhauer">Johannes C. Fleischhauer</name></noRegion><name sortKey="Berger, Wolfgang" sort="Berger, Wolfgang" uniqKey="Berger W" first="Wolfgang" last="Berger">Wolfgang Berger</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Haralambous, Elene" sort="Haralambous, Elene" uniqKey="Haralambous E" first="Elene" last="Haralambous">Elene Haralambous</name></region><name sortKey="Bitner Glindzicz, Maria" sort="Bitner Glindzicz, Maria" uniqKey="Bitner Glindzicz M" first="Maria" last="Bitner-Glindzicz">Maria Bitner-Glindzicz</name><name sortKey="Mckay, Gareth J" sort="Mckay, Gareth J" uniqKey="Mckay G" first="Gareth J" last="Mckay">Gareth J. Mckay</name><name sortKey="Saihan, Zubin" sort="Saihan, Zubin" uniqKey="Saihan Z" first="Zubin" last="Saihan">Zubin Saihan</name><name sortKey="Silvestri, Giuliana" sort="Silvestri, Giuliana" uniqKey="Silvestri G" first="Giuliana" last="Silvestri">Giuliana Silvestri</name><name sortKey="Webster, Andrew R" sort="Webster, Andrew R" uniqKey="Webster A" first="Andrew R" last="Webster">Andrew R. Webster</name></country><country name="Belgique"><noRegion><name sortKey="De Baere, Elfride" sort="De Baere, Elfride" uniqKey="De Baere E" first="Elfride" last="De Baere">Elfride De Baere</name></noRegion><name sortKey="Kremer, Hannie" sort="Kremer, Hannie" uniqKey="Kremer H" first="Hannie" last="Kremer">Hannie Kremer</name><name sortKey="Leroy, Bart P" sort="Leroy, Bart P" uniqKey="Leroy B" first="Bart P" last="Leroy">Bart P. Leroy</name></country><country name="Canada"><region name="Québec"><name sortKey="Koenekoop, Robert K" sort="Koenekoop, Robert K" uniqKey="Koenekoop R" first="Robert K" last="Koenekoop">Robert K. Koenekoop</name></region></country><country name="Espagne"><noRegion><name sortKey="Millan, Jose M" sort="Millan, Jose M" uniqKey="Millan J" first="Jose M" last="Millan">Jose M. Millan</name></noRegion></country><country name="Danemark"><noRegion><name sortKey="Rosenberg, Thomas" sort="Rosenberg, Thomas" uniqKey="Rosenberg T" first="Thomas" last="Rosenberg">Thomas Rosenberg</name></noRegion></country><country name="Finlande"><region name="Uusimaa"><name sortKey="Joensuu, Tarja" sort="Joensuu, Tarja" uniqKey="Joensuu T" first="Tarja" last="Joensuu">Tarja Joensuu</name></region><name sortKey="Sankila, Eeva Marja" sort="Sankila, Eeva Marja" uniqKey="Sankila E" first="Eeva-Marja" last="Sankila">Eeva-Marja Sankila</name></country><country name="France"><noRegion><name sortKey="Weil, Dominique" sort="Weil, Dominique" uniqKey="Weil D" first="Dominique" last="Weil">Dominique Weil</name></noRegion></country><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Wissinger, Bernd" sort="Wissinger, Bernd" uniqKey="Wissinger B" first="Bernd" last="Wissinger">Bernd Wissinger</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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